Abstract: The classical statistical diffusion theory and the observed spectral properties are used to estimate the Kolmogorov constant C₀ under different stability conditions and for the three velocity components. Such C₀ values are realted to the characteriscs of the Eulerian velocity spectra and to the ratio between the Lagrangian and the Eulerian statistics. The results of this new method of deriving C₀ values are shown to be in good agreement with previously determined C₀ values in the Planetary Boundary Layer. The C₀ changes as a function of the energy-containing eddies for each turbulent flow, according to the Landau arguments.